In Operation: GALACSIA, he was introduced when Star Wolf caught Dib Membrane in their territory, so Rattlesnake Jake was sent to attack him, which he succeeded. When they were called over to the Tallest, Jake shot up and snatched away Purple's candy bar, eating it. He was bested by Dib Membrane when they went to fight them, and was later at Planet Food Courtia, demanding some rat guts, which they didn't have. When Star Wolf got into that little fight with Star Fox on Planet Corneria, Jake fought Slippy.
When Nigel went to Nightmare Land, he went into Jake's greatest fear in the Hall of Fear, and his greatest fear was birds. When Nigel and the team fought Star Wolf again, Katie flew by and helped them beat Jake by projecting a holographic image of a hawk. But while Jake is afraid of birds, he didn't seem afraid of Falco in any way. He and Star Wolf attacked Moonbase during The Invasion, in which Jake bit Fanny's leg. On Earth, he was tied into a knot by Sonya after trying to shoot and kill Sector W. Jake then dragged Rachel to the Tallest when they reached the Massive, and tried squeeezing the life out of her, but Nigel arrived just in time. After Ava betrayed the team and handed Red and Purple the Stars, Jake and Star Wolf were betrayed as well and locked away. When they broke out and destroyed the Massive, Jake dragged the Tallest to their doom.
Jake and Star Wolf helped the main group during The Grande Finale. When Nigel defeated Dimentia, Rattlesnake Jake "tipped his hat to him", meaning he now (partially) respects Nigel.
In Truth or Dare: KND Plus Others, Jake and Star Wolf were in the audience during the Bonus Showdown, where Jake was rooting for Nagini.
In The Great Galactic Race, Jake shot off enemies in their Landcrusher before they and Star Wolf crashed into a tree on Planet Flora. Jake is immune to Nova's telepathy due to him being an intelligent animal.
In the one-shot "Noah's Nightmare", Noah had a nightmare where Boba Fett carried her over to Jake for him to eat her out of revenge to Buddy for killing Nagini.